Browsing by Author "Pocetti, Florencia"

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    Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization
    (2018) Sözer, Esin B.; Pocetti, Florencia; Vernier, P. Thomas
    "Imaging of fluorescent small molecule transport into electropermeabilized cells reveals polarized patterns of entry, which must reflect in some way the mechanisms of the migration of these molecules across the compromised membrane barrier. In some reports, transport occurs primarily across the areas of the membrane nearest the positive electrode (anode), but in others cathode-facing entry dominates. Here we compare YO-PRO-1, propidium, and calcein uptake into U-937 cells after nanosecond (6 ns) and microsecond (220 ls) electric pulse exposures. Each of the three dyes exhibits a different pattern. Calcein shows no preference for anode- or cathode-facing entry that is detectable with our measurement system. Immediately after a microsecond pulse, YO-PRO-1 and propidium enter the cell roughly equally from the positive and negative poles, but transport through the cathode-facing side dominates in less than 1 s. After nanosecond pulse permeabilization, YO-PRO-1 and propidium enter primarily on the anode-facing side of the cell."
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    Transport of charged small molecules after electropermeabilization — drift and diffusion
    (2018-03) Sözer, Esin B.; Pocetti, Florencia; Vernier, P. Thomas
    "We provide a direct quantitative comparison of molecular transport of three similarly sized but chemically different fluorescent indicators of membrane permeabilization — two cationic molecules (YO-PRO-1 and propidium), and one anionic molecule (calcein). Our results show that the influx of YO-PRO-1 and propidium (both cations) into electropermeabilized cells is an order of magnitude greater than that of calcein (an anion) after the same pulse exposure. Calcein efflux from cells loaded before permeabilization, however, is similar in magnitude to YO-PRO-1 and propidium influx."