Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization

Sözer, Esin  B.; Pocetti, Florencia; Vernier, P. Thomas

Artículo de Publicación Periódica:
Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization

dc.contributor.author	Sözer, Esin B.
dc.contributor.author	Pocetti, Florencia
dc.contributor.author	Vernier, P. Thomas
dc.date.accessioned	2019-07-11T20:23:20Z
dc.date.available	2019-07-11T20:23:20Z
dc.date.issued	2018
dc.description.abstract	"Imaging of fluorescent small molecule transport into electropermeabilized cells reveals polarized patterns of entry, which must reflect in some way the mechanisms of the migration of these molecules across the compromised membrane barrier. In some reports, transport occurs primarily across the areas of the membrane nearest the positive electrode (anode), but in others cathode-facing entry dominates. Here we compare YO-PRO-1, propidium, and calcein uptake into U-937 cells after nanosecond (6 ns) and microsecond (220 ls) electric pulse exposures. Each of the three dyes exhibits a different pattern. Calcein shows no preference for anode- or cathode-facing entry that is detectable with our measurement system. Immediately after a microsecond pulse, YO-PRO-1 and propidium enter the cell roughly equally from the positive and negative poles, but transport through the cathode-facing side dominates in less than 1 s. After nanosecond pulse permeabilization, YO-PRO-1 and propidium enter primarily on the anode-facing side of the cell."	en
dc.identifier.issn	0022-2631
dc.identifier.uri	http://ri.itba.edu.ar/handle/123456789/1644
dc.language.iso	en	en
dc.relation	info:eu-repo/semantics/reference/doi/10.1007/s00232-017-9962-1
dc.relation	info:eu-repo/grantAgreement/AFOSR/FA9550-14-1-0123/US. Arlington, VA.
dc.relation	info:eu-repo/grantAgreement/AFOSR/MURI/FA9550-15-1-0517/US. Arlington, VA.
dc.relation	info:eu-repo/grantAgreement/MECCyT/BEC.AR/AR.Ciudad Autónoma de Buenos Aires
dc.rights	https://creativecommons.org/licenses/by/4.0/
dc.subject	MOLECULAS	es
dc.subject	TRANSPORTE	es
dc.subject	ELECTROPORACION	es
dc.subject	MEMBRANAS	es
dc.title	Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization	en
dc.type	Artículos de Publicaciones Periódicas	es
dc.type	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Artículo de Publicación Periódica
itba.description.filiation	Fil: Pocetti, Florencia. Instituto Tecnológico de Buenos Aires; Argentina.
itba.description.filiation	Fil: Sözer, Esin B. Old Dominion University; Estados Unidos.
itba.description.filiation	Fil: Vernier, P. Thomas. Old Dominion University; Estados Unidos.